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Viral Delivery

Viral Delivery Expression

Advanced viral vector development and gene delivery systems for efficient gene transfer and therapeutic applications.

Overview

Viral vectors are the most efficient tools for gene delivery into mammalian cells. NTSR provides expert services in the design, packaging, and purification of high-titer Lentivirus (LV) and Adeno-Associated Virus (AAV). Our platform is optimized for both research-grade and high-purity applications, ensuring robust gene expression across a wide variety of cell types, including hard-to-transfect primary cells and neurons.

Vector Systems

Lentivirus (LV)

Integrative delivery for stable, long-term expression. Ideal for dividing and non-dividing cells.

AAV (Serotypes 1-9)

Non-integrative episomal expression. Wide range of serotypes for tissue-specific targeting in vivo.

Retrovirus

Specialized for stable integration into actively dividing cell populations (e.g., stem cells).

Quality Control Standards

Parameter Lentivirus (LV) AAV (1-9) Verification Method
Unpurified Titer 10^6 - 10^7 TU/mL 10^9 - 10^{10} GC/mL qPCR / p24 ELISA
Purified Titer 10^8 - 10^9 TU/mL 10^{12} - 10^{13} GC/mL qPCR / FACS
Purity Level Ultracentrifuged Gradient Purified SDS-PAGE / Endotoxin
Safety Check RCL Testing Mycoplasma Free Sterility Assay

Key Features

  • High-titer, high-purity preparations
  • Custom transfer vector design
  • Serotype-specific tropism optimization

Applications

  • In vivo gene therapy research
  • Stable cell line generation
  • CRISPR/Cas9 delivery systems
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