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Protein Purification

Protein Purification

Advanced protein purification services using chromatography, filtration, and specialized techniques for high-purity protein isolation.

Overview

Protein purification is the process of isolating a specific protein of interest from a complex biological mixture. NTSR specializes in multi-step purification strategies designed to achieve ultra-high purity while maintaining biological activity. Using state-of-the-art FPLC systems and a variety of resin chemistries (Affinity, Ion-Exchange, Size-Exclusion), we deliver proteins suitable for structural biology, drug screening, and therapeutic development.

Purification Methodologies

Affinity Chromatography

Highly selective isolation using tags (His, GST, Flag) or specific ligands for maximum initial purity.

Ion-Exchange (IEX)

Separation based on surface charge using Cation (CM/SP) or Anion (DEAE/Q) exchange resins.

SEC / Gel Filtration

Size-Exclusion Chromatography for polishing and removing aggregates to ensure monodispersity.

Purity & Quality Standards

Parameter Trial Grade Analysis Grade Crystallography Grade
Purity (SDS-PAGE) >75% >90% >98%
Endotoxin Level N/A < 1 EU/mg < 0.1 EU/mg
Scale Range µg - mg mg - grams Custom Bulk
QC Verification BCA + PAGE LC-MS + SEC Activity Assay + NMR

Key Features

  • Customizable purification protocols
  • Tag removal (TEV, Enterokinase)
  • Refolding services for inclusion bodies

Applications

  • X-ray Crystallography
  • High-Throughput Screening
  • Pre-clinical animal studies
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