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Gene Synthesis

Gene Synthesis

Custom DNA synthesis with advanced assembly technologies including Gibson Assembly and Golden Gate for complex genetic constructs.

Overview

NTSR provides industry-leading gene synthesis services, leveraging advanced proprietary algorithms for sequence design and optimized assembly protocols. Our process ensures 100% sequence accuracy through NGS-based verification, catering to complex genetic constructs that other providers might find challenging. Whether it's high-GC content, repetitive sequences, or large multigene clusters, our platform delivers high-fidelity results ready for downstream applications.

Synthesis Types

Standard Synthesis

Ideal for routine sequences up to 5kb with standard GC content. Fast turnaround and cost-effective.

Complex Genes

Designed for sequences with extreme GC content (>70% or <30%), long repeats, or secondary structures.

Large Constructs

Custom assembly of gene clusters and pathways up to 20kb using Gibson or Golden Gate methods.

Technical Specifications

Feature Economy Grade Standard Grade Premium Grade
Sequence Length < 1kb 1kb - 5kb Up to 20kb
Accuracy 99.9% 100% Verified Error-Free (NGS)
Turnaround Time 12-15 Days 8-10 Days 5-7 Days (Express)
Deliverable Lyophilized DNA DNA + Map Glycerol Stock

Key Features

  • Free AI-based Codon Optimization
  • Subcloning into any vector
  • Sequence verification included

Applications

  • Vaccine development
  • CRISPR gene editing
  • Synthetic biology
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